Sample Preparation for NGS – A Comprehensive Guide
Aug 21, 2024 · Common challenges in sample preparation. Challenge 1: Many samples are extracted from a limited number of cells – or even a single cell.These don’t provide enough genetic material alone and so need to undergo PCR. However, this amplification step is prone to introducing bias to the sample.
DNA Sequencing: How to Choose the Right Technology
Aug 6, 2021 · The 2024 Sequencing Buyer’s Guide. Introduction. It has been over 30 years since the first generation of DNA sequencing technology was developed in 1977. Since then, sequencing platforms have made considerable progress and every transformation has led to a huge shift towards furthering genome research, clinical disease research and drug development.
What is multiplex PCR? - Front Line Genomics
Jul 26, 2021 · The polymerase chain reaction (PCR) is a well-established amplification technique that is widely used to rapidly make millions of copies of a specific DNA sample. It was developed by Kary Mullis in the 1980’s and is based on the ability of DNA polymerase to synthesize a new strand of complementary DNA from a template strand.
A brief history of Next Generation Sequencing (NGS)
Jul 26, 2021 · Join David Smith (Mayo Clinic), Shawn Baker (SanDiegOmics) and Tiffany Boughtwood (Australian Genomics) as they discuss how the upcoming shifts within the NGS market could affect genomics research in the future and what impact it may have on healthcare around the world: Exploring the Current Sequencing Landscape The history of DNA sequencing
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Unlocking the future: where is gene editing going next?
Jun 6, 2023 · In our latest feature, we explore the future of gene editing and the challenges we must overcome to harness its full potential.
Everything you need (and want) to know about tardigrades
Oct 18, 2022 · By now, most of us have heard of the extremely hardy (and cute) tardigrade, otherwise known as the water bear or moss piglet. These microscopic animals have been subjected to some torturous conditions in a bid to understand how (and why) they are able to survive in such harsh environments.
Long-read sequencing vs short-read sequencing
Jul 26, 2021 · Join David Smith (Mayo Clinic), Shawn Baker (SanDiegOmics) and Tiffany Boughtwood (Australian Genomics) as they discuss how the upcoming shifts within the NGS market could affect genomics research in the future and what impact it may have on healthcare around the world: Exploring the Current Sequencing …
How-to: perform a genome-wide association study (GWAS)
Sep 10, 2024 · Genome-wide association studies are commonly used in the genomics world to identify variants associated with a trait or disease.During a GWAS, researchers will identify areas of the genome that show a strong signal linked to the trait in question; that is, in the discovery cohort, areas or specific markers in which variation is more common than in the controls.
A longstanding mystery: How do identical twins arise?
Oct 1, 2021 · Scientists have discovered DNA methylation differences that are unique to identical twins, helping to unravel the exact molecular events that lead to division of a zygote.. Monozygotic twins, or identical twins, result from the fertilisation of a single egg with a single sperm.At some point during the very early pre-implantation stages of …