To construct the library, we performed two separate 200 μl Golden Gate reactions that each contained 200 fmol of destination vector and each cassette, and pooled the DNA for use in a single …

Sep 26, 2023 · We designed and created a DNA library encoding 1.7 × 106 unique protein sequences. This new library of stable de novo α-helical proteins is well suited for screens and selections for a …

Nov 1, 2021 · By using Type IIS enzymes in a format like BioBricks, we have enabled a faster and efficient assembly with reduced scarring, which performs at a similarly fast pace as Golden Gate, but …

Programming of DNA assembly by geometry is generally applicable. To demonstrate the generalizability of this technique, we tested it on two other systems. The first system is a coassembly of two …

Although there have been only a few reports, it has been demonstrated that DNA may act as a potent ligand to construct arti ficial cellular clusters or microtis-sues toward a variety of biological applications.

NSF Public Access Search Results Integrated computer-aided engineering and design for DNA assemblies Citation Details

The Cello library used in the Cello project consists of 12 orthogonal Tet repressors that drive cassettes including promoters, coding sequences, and terminators to function as NOT/NOR gates.

– Engineering Biology Roadmap 2019 GENE EDITING, SYNTHESIS, AND ASSEMBLY Manufacture thousands of very long oligonucleotides with high fidelity. Many-fragment DNA assembly with …

Surface-assisted, tile-based DNA self-assembly is a powerful method to construct large, two-dimensional (2D) nanoarrays. To further increase the structural complexity, one idea is to incorporate …

These features combine to make RNA a highly versatile molecule, endowed with immense conformational variability. This renders the prediction of RNA secondary and particularly tertiary …